5-methylcytosine modification of an Epstein-Barr virus noncoding RNA decreases its stability.

ABSTRACT: Many cellular noncoding RNAs contain chemically modified nucleotides that are essential for their function. The Epstein-Barr virus expresses two highly abundant noncoding RNAs called EBV-encoded RNA 1 (EBER1) and EBER2. To examine whether these viral RNAs contain modified nucleotides, we purified native EBERs from EBV-infected cells and performed mass spectrometry analysis. While EBER2 contains no modified nucleotides at stoichiometric amounts, EBER1 was found to carry 5-methylcytosine (m⁵C) modification. Bisulfite sequencing indicated that a single cytosine of EBER1 is methylated in ∼95% of molecules, and the RNA methyltransferase NSUN2 was identified as the EBER1-specific writer. Intriguingly, ablation of NSUN2 and thus loss of m⁵C modification resulted in an increa

SUBMITTER: Henry BA 

PROVIDER: S-EPMC7373997 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature