Impact of PepT1 deletion on microbiota composition and colitis requires multiple generations.

ABSTRACT: Numerous studies of knockout mice find impacts on microbiota composition that influence host phenotype. However, such differences can vanish when KO mice are compared directly to WT littermates, suggesting these differences do not reflect the genetic deletion per se but microbiota composition drifting over generations. Hence, our hypothesis that absence of di/tri-peptide transporter PepT1 altered microbiota composition resulting in resistance to colitis compelled scrutiny. In this study, we used PepT1^-/- and WT founder mice bred separately for multiple generations. Such mice were then bred to each other to generate F1 PepT1^-/- and WT littermates, which were then bred within their genotype to generate F2, F3, and F4, offspring. Here we report that founder PepT1^-/-

SUBMITTER: Viennois E 

PROVIDER: S-EPMC7374158 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature