Multiomics global landscape of stemness-related gene clusters in adipose-derived mesenchymal stem cells.

ABSTRACT:

Background

Adipose-derived mesenchymal stem cells (AD-MSCs) are a type of stem cell that is abundant and widely used. The molecular characteristics of AD-MSCs from different passages from donors of different ages have not been well elucidated.

Methods

Six kinds of AD-MSCs ((E1, E2, E3, Y1, Y2, and Y3) with E denoting cells derived from an elderly patient, Y denoting cells derived from a young patient, and 1, 2, and 3 representing passages 3, 6, and 10) were obtained from human abdominal adipose tissue. We obtained the protein expression profile, the mRNA expression profile, the lncRNA expression profile, and the methylation profile of each kind of AD-MSC by sequencing. After calculating the stemness indices, genes related to stemness were extracted. The multiomics correlatio

SUBMITTER: Lu GM 

PROVIDER: S-EPMC7374825 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature