METTL1-mediated m⁷G methylation maintains pluripotency in human stem cells and limits mesoderm differentiation and vascular development.

ABSTRACT:

Background

7-Methylguanosine (m⁷G) is one of the most conserved modifications in nucleosides within tRNAs and rRNAs. It plays essential roles in the regulation of mRNA export, splicing, and translation. Recent studies highlighted the importance of METTL1-mediated m⁷G tRNA methylome in the self-renewal of mouse embryonic stem cells (mESCs) through its ability to regulate mRNA translation. However, the exact mechanisms by which METTL1 regulates pluripotency and differentiation in human induced pluripotent stem cells (hiPSCs) remain unknown. In this study, we evaluated the functions and underlying molecular mechanisms of METTL1 in regulating hiPSC self-renewal and differentiation in vivo and in vitro.

Methods

By establishing METTL1 knockdown (KD) hiPSCs, g

SUBMITTER: Deng Y 

PROVIDER: S-EPMC7374972 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature