Bone Marrow Endothelial Cells Regulate Myelopoiesis in Diabetes Mellitus.

ABSTRACT:

Background

Diabetes mellitus is a prevalent public health problem that affects about one-third of the US population and leads to serious vascular complications with increased risk for coronary artery disease. How bone marrow hematopoiesis contributes to diabetes mellitus complications is incompletely understood. We investigated the role of bone marrow endothelial cells in diabetic regulation of inflammatory myeloid cell production.

Methods

In 3 types of mouse diabetes mellitus, including streptozotocin, high-fat diet, and genetic induction using leptin-receptor-deficient db/db mice, we assayed leukocytes, hematopoietic stem and progenitor cells (HSPC). In addition, we investigated bone marrow endothelial cells with flow cytometry and expression profiling.

Results

In d

SUBMITTER: Hoyer FF 

PROVIDER: S-EPMC7375017 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature