Suppression of cardiomyocyte functions by β-CTX isolated from the Thai king cobra (Ophiophagus hannah) venom via an alternative method.

ABSTRACT:

Background

Beta-cardiotoxin (β-CTX), the three-finger toxin isolated from king cobra (Ophiophagus hannah) venom, possesses β-blocker activity as indicated by its negative chronotropy and its binding property to both β-1 and β-2 adrenergic receptors and has been proposed as a novel β-blocker candidate. Previously, β-CTX was isolated and purified by FPLC. Here, we present an alternative method to purify this toxin. In addition, we tested its cytotoxicity against different mammalian muscle cell types and determined the impact on cardiac function in isolated cardiac myocyte so as to provide insights into the pharmacological action of this protein.

Methods

β-CTX was isolated from the crude venom of the Thai king cobra using reverse-phased and cation exchange HPLC. In vi

SUBMITTER: Lertwanakarn T 

PROVIDER: S-EPMC7375408 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature