Endothelial-Initiated Crosstalk Regulates Dental Pulp Stem Cell Self-Renewal.

ABSTRACT: Interactions with the microenvironment modulate the fate of stem cells in perivascular niches in tissues (e.g., bone) and organs (e.g., liver). However, the functional relevance of the molecular crosstalk between endothelial cells and stem cells within the perivascular niche in dental pulps is unclear. Here, we tested the hypothesis that endothelial cell-initiated signaling is necessary to maintain self-renewal of dental pulp stem cells. Confocal microscopy showed that ALDH1^high and Bmi-1^high stem cells are preferentially localized in close proximity to blood vessels in physiological human dental pulps. Secondary orosphere assays revealed that endothelial cell-derived factors (e.g., interleukin-6 [IL-6]) promote self-renewal of dental pulp stem cells cultured in low-a

SUBMITTER: Oh M 

PROVIDER: S-EPMC7375737 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature