Charcot-Leyden crystal protein/galectin-10 interacts with cationic ribonucleases and is required for eosinophil granulogenesis.

ABSTRACT:

Background

The human eosinophil Charcot-Leyden crystal (CLC) protein is a member of the Galectin superfamily and is also known as galectin-10 (Gal-10). CLC/Gal-10 forms the distinctive hexagonal bipyramidal crystals that are considered hallmarks of eosinophil participation in allergic responses and related inflammatory reactions; however, the glycan-containing ligands of CLC/Gal-10, its cellular function(s), and its role(s) in allergic diseases are unknown.

Objective

We sought to determine the binding partners of CLC/Gal-10 and elucidate its role in eosinophil biology.

Methods

Intracellular binding partners were determined by ligand blotting with CLC/Gal-10, followed by coimmunoprecipitation and coaffinity purifications. The role of CLC/Gal-10 in eosinophil function w

SUBMITTER: Grozdanovic MM 

PROVIDER: S-EPMC7375938 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature