Viable SARS-CoV-2 in various specimens from COVID-19 patients.

ABSTRACT:

Objectives

The aim was to determine whether various clinical specimens obtained from COVID-19 patients contain the infectious virus.

Methods

To demonstrate whether various clinical specimens contain the viable virus, we collected naso/oropharyngeal swabs and saliva, urine and stool samples from five COVID-19 patients and performed a quantitative polymerase chain reaction (qPCR) to assess viral load. Specimens positive with qPCR were subjected to virus isolation in Vero cells. We also used urine and stool samples to intranasally inoculate ferrets and evaluated the virus titres in nasal washes on 2, 4, 6 and 8 days post infection.

Results

SARS-CoV-2 RNA was detected in all naso/oropharyngeal swabs and saliva, urine and stool samples collected between days 8 and 30 of th

SUBMITTER: Jeong HW 

PROVIDER: S-EPMC7375961 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature