CALX-CBD1 Ca²⁺-Binding Cooperativity Studied by NMR Spectroscopy and ITC with Bayesian Statistics.

ABSTRACT: The Na⁺/Ca²⁺ exchanger of Drosophila melanogaster, CALX, is the main Ca²⁺-extrusion mechanism in olfactory sensory neurons and photoreceptor cells. Na⁺/Ca²⁺ exchangers have two Ca²⁺ sensor domains, CBD1 and CBD2. In contrast to the mammalian homologs, CALX is inhibited by Ca²⁺ binding to CALX-CBD1, whereas CALX-CBD2 does not bind Ca²⁺ at physiological concentrations. CALX-CBD1 consists of a β-sandwich and displays four Ca²⁺-binding sites at the tip of the domain. In this study, we used NMR spectroscopy and isothermal titration calorimetry (ITC) to investigate the cooperativity of Ca²⁺ binding to CALX-CBD1. We observed that this domain binds Ca²⁺ in the slow exchange regi

SUBMITTER: Cardoso MVC 

PROVIDER: S-EPMC7376077 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature