Retinoic acid receptor γ activation promotes differentiation of human induced pluripotent stem cells into esophageal epithelium.

ABSTRACT:

Background

The esophagus is known to be derived from the foregut. However, the mechanisms regulating this process remain unclear. In particular, the details of the human esophagus itself have been poorly researched. In this decade, studies using human induced pluripotent stem cells (hiPSCs) have proven powerful tools for clarifying the developmental biology of various human organs. Several studies using hiPSCs have demonstrated that retinoic acid (RA) signaling promotes the differentiation of foregut into tissues such as lung and pancreas. However, the effect of RA signaling on the differentiation of foregut into esophagus remains unclear.

Methods

We established a novel stepwise protocol with transwell culture and an air-liquid interface system for esophageal epithelial cell

SUBMITTER: Koterazawa Y 

PROVIDER: S-EPMC7376085 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature