Connecting Coil-to-Globule Transitions to Full Phase Diagrams for Intrinsically Disordered Proteins.

ABSTRACT: Phase separation is thought to underlie spatial and temporal organization that is required for controlling biochemical reactions in cells. Multivalence of interaction motifs, also known as stickers, is a defining feature of proteins that drive phase separation. Intrinsically disordered proteins with stickers uniformly distributed along the linear sequence can serve as scaffold molecules that drive phase separation. The sequence-intrinsic contributions of disordered proteins to phase separation can be discerned by computing or measuring sequence-specific phase diagrams. These help to delineate the combinations of protein concentration and a suitable control parameter, such as temperature, that support phase separation. Here, we present an approach that combines detailed simulations with a n

SUBMITTER: Zeng X 

PROVIDER: S-EPMC7376131 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature