A pooled genome-wide screening strategy to identify and rank influenza host restriction factors in cell-based vaccine production platforms.

ABSTRACT: Cell-derived influenza vaccines provide better protection and a host of other advantages compared to the egg-derived vaccines that currently dominate the market, but their widespread use is hampered by a lack of high yield, low cost production platforms. Identification and knockout of innate immune and metabolic restriction factors within relevant host cell lines used to grow the virus could offer a means to substantially increase vaccine yield. In this paper, we describe and validate a novel genome-wide pooled CRISPR/Cas9 screening strategy that incorporates a reporter virus and a FACS selection step to identify and rank restriction factors in a given vaccine production cell line. Using the HEK-293SF cell line and A/PuertoRico/8/1934 H1N1 influenza as a model, we identify 64 putative infl

SUBMITTER: Sharon DM 

PROVIDER: S-EPMC7376217 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature