Multigenic engineering of the chloroplast genome in the green alga Chlamydomonas reinhardtii.

ABSTRACT: The chloroplast of microalgae such as Chlamydomonas reinhardtii represents an attractive chassis for light-driven production of novel recombinant proteins and metabolites. Methods for the introduction and expression of transgenes in the chloroplast genome (=plastome) of C. reinhardtii are well-established and over 100 different proteins have been successfully produced. However, in almost all reported cases the complexity of the genetic engineering is low, and typically involves introduction into the plastome of just a single transgene together with a selectable marker. In order to exploit fully the potential of the algal chassis it is necessary to establish methods for multigenic engineering in which many transgenes can be stably incorporated into the plastome. This would all

SUBMITTER: Larrea-Alvarez M 

PROVIDER: S-EPMC7376270 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature