Nitrogen mustard-induced corneal injury involves the sphingomyelin-ceramide pathway.

ABSTRACT:

Purpose

Nitrogen mustard (NM), which simulates the effects of sulfur mustard (SM), is a potent vesicant known to cause irreversible corneal damage. This study investigates the mechanisms by which NM induces corneal damage by examining the impact of NM exposure on the morphology and lipidome of the cornea.

Methods

Intact ex vivo rabbit eyes were placed in serum-free DMEM organ culture. NM (0, 1, 2.5, 5 or 10 mg/ml) was applied to the central cornea for 5, 10 or 15 min using a 5 mm filter disk and subsequently rinsed with DMEM. Corneas were then cultured for 3, 24, or 48 h before being fixed for morphological analysis or for 24 h before being snap frozen for lipidomic analysis.

Results

No morphological changes were detected 3 h after NM exposure. Twenty-four h after exp

SUBMITTER: Charkoftaki G 

PROVIDER: S-EPMC7376578 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature