Identification and antigenicity of the Babesia caballi spherical body protein 4 (SBP4).

ABSTRACT:

Background

The tick-borne intra-erythrocytic apicomplexan Babesia caballi is one of the etiological agents of equine babesiosis, an economically important disease of equids in most tropical and subtropical areas of the world. Discovering candidate antigens for improved diagnostic tools and vaccines remains needed for controlling equine babesiosis. This study describes the B. caballi sbp4 (Bcsbp4) gene and protein (BcSBP4) and analyzes its antigenicity in infected equids.

Methods

BLAST searches of an uncurated B. caballi assembly genome using the B. bovis SBP4 as a query were carried out, followed by PCR amplification and sequencing of a newly identified BcSBP4. Characterization of this novel gene and protein was performed by bioinformatics analysis, western blots, immunofluo

SUBMITTER: Mahmoud MS 

PROVIDER: S-EPMC7376649 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature