Reduced polyfunctional T cells and increased cellular activation markers in adult allergy patients reporting adverse reactions to food.

ABSTRACT:

Background

The underlying cellular mechanisms causing adverse reactions to food are complex and still not fully understood. Therefore, in this study we aimed to identify functional and/or phenotypical immune cell signatures characteristic for adult patients reporting adverse reactions to food. By mass cytometry, we performed high-dimensional profiling of peripheral blood mononuclear cells (PBMC) from adult patients reporting adverse reactions to food and healthy controls. The patients were grouped according to sIgE-positive or sIgE-negative serology to common food and inhalant allergens. Two broad antibody panels were used, allowing determination of major immune cell populations in PBMC, as well as activation status, proliferation status, and cytokine expression patterns after PMA/

SUBMITTER: Sonnet F 

PROVIDER: S-EPMC7376650 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature