A novel knock out strategy to enhance recombinant protein expression in Escherichia coli.

ABSTRACT:

Background

The expression of recombinant proteins triggers a stress response which downregulates key metabolic pathway genes leading to a decline in cellular health and feedback inhibition of both growth and protein expression. Instead of individually upregulating these downregulated genes or improving transcription rates by better vector design, an innovative strategy would be to block this stress response thereby ensuring a sustained level of protein expression.

Results

We postulated that the genes which are commonly up-regulated post induction may play the role of signalling messengers in mounting the cellular stress response. We identified those genes which have no known downstream regulatees and created knock outs which were then tested for GFP expression. Many of these

SUBMITTER: Sharma AK 

PROVIDER: S-EPMC7376861 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature