Lignocellulosic pretreatment-mediated phenolic by-products generation and their effect on the inhibition of an endo-1,4-β-xylanase from Thermomyces lanuginosus VAPS-24.

ABSTRACT: The inhibitory effect of eight model lignin derivatives (ferulic acid, guaiacol, kraft lignin (alkali, low sulfonate content), p-coumaric acid, gallic acid, syringic acid, vanillin and vanillic acid) on XynA activity was evaluated. The model lignin derivatives viz. gallic acid, vanillic acid and vanillin were inhibitory to XynA activity, with an over 50% reduction in activity at concentrations as low as 0.5 mg/ml. However, enzyme deactivation studies in the absence of substrate showed that these pretreatment by-products do not interact with the enzyme except when in the presence of its substrate. The effect of the main structural properties of the pretreatment-derived phenolics, for example their hydroxyl and carbonyl group types, on XynA enzyme inhibition was investigated. The pres

SUBMITTER: Mathibe BN 

PROVIDER: S-EPMC7377001 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature