Establishment of C₂₀Mab-11, a novel anti-CD20 monoclonal antibody, for the detection of B cells.

ABSTRACT: CD20 is one of several B-lymphocyte antigens that has been shown to be an effective target for the detection and treatment of B-cell lymphomas. Sensitive and specific monoclonal antibodies (mAbs) are required for every application used for the diagnosis of B-cell lymphoma. Although many anti-CD20 mAbs have been established, the types of applications, those anti-CD20 can be used in, are limited. In this study, we aimed to establish novel anti-CD20 mAbs to be used for broad applications, such as flow cytometry, western blot, and immunohistochemical analyses, using the Cell-Based Immunization and Screening (CBIS) method. One of the established mAbs, C₂₀Mab-11 (IgM, kappa), detected overexpression of CD20 in CHO-K1 or LN229 cell lines, indicating that C₂₀Mab-11 is specifi

SUBMITTER: Furusawa Y 

PROVIDER: S-EPMC7377059 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature