Kinetics of Fast Tetramerization of the Huntingtin Exon 1 Protein Probed by Concentration-Dependent On-Resonance <i>R</i>_1ρ Measurements.

ABSTRACT: An approach for the quantitative description of the kinetics of very fast exchange processes (τ_ex < 50-100 μs) associated with transient, reversible protein oligomerization, is presented. We show that on-resonance ¹⁵N-R_1ρ measurements conducted as a function of protein concentration at several spin-lock radio frequency field strengths are indispensable for unambiguous determination of the rate constants for interconversion between monomeric and higher order oligomeric species. The approach is experimentally demonstrated on the study of fast, reversible tetramerization of the full-length Huntingtin exon 1 protein, htt^ex1, responsible for Huntington's disease. Incorporation of concentration-dependent ¹⁵N-R_2,eff d

SUBMITTER: Ceccon A 

PROVIDER: S-EPMC7377354 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature