Fluorescence in situ hybridization in species-specific diagnosis of ovine Campylobacter abortions.

ABSTRACT: Campylobacter infection is a leading cause of ovine abortion worldwide. Campylobacter fetus and C. jejuni are the major species involved. We report herein on abortion storms in 4 Danish sheep flocks. Initially, no pathogenic bacteria were isolated from placental and fetal tissues on aerobic and selective media despite the presence of severe suppurative and necrotizing placentitis with numerous bacteria located intracellularly in trophoblasts. Fluorescence in situ hybridization (FISH) was then applied on abortion material from 13 cases; species-specific oligonucleotide probes directed against either C. fetus or C. jejuni were used in combination with a general bacterial probe. C. fetus was detected as the only lesion-associated bacterial species in

SUBMITTER: Wolf-Jackel GA 

PROVIDER: S-EPMC7377615 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature