Validation of a quantitative PCR assay for the detection of 2 Flavobacterium columnare genomovars.

ABSTRACT: Flavobacterium columnare is the causative agent of columnaris disease in a variety of fish hosts. Using modifications to previously established protocols, a quantitative PCR (qPCR) assay was validated for the detection of 2 predominant F. columnare genomovars. The oligonucleotide primer and probe combination was designed to amplify a 203-bp region of the chondroitin AC lyase gene (GenBank AY912281) of F. columnare. There were no significant differences in amplification between genomovars. Comparable quantities of genomic DNA from 10 F. columnare strains, 5 representatives of each genomovar, produced similar results. Serial dilutions of purified PCR product demonstrated the limit of sensitivity for the assay was ~ 10 copies per reaction. The presence of gill and

SUBMITTER: Gibbs GD 

PROVIDER: S-EPMC7377616 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature