Metformin protects against mouse oocyte apoptosis defects induced by arecoline.

ABSTRACT:

Objectives

Arecoline is the main bioactive substance extracted from Areca catechu L, which has cell, neural and genetic toxicity. The function of arecoline in reproductive system has not been well explored.

Materials and methods

To investigate the toxic effects of arecoline on oocyte development, immunofluorescence staining, qPCR, Western blotting, sperm binding assays and in vitro fertilization were performed to evaluate oocyte meiosis competence and embryo development.

Results

Our data revealed that arecoline exposure disrupts actin filament dynamics, spindle assembly and kinetochore-microtubule attachment stability in mouse oocytes, leading to aneuploidy and oocyte meiosis arrest. In addition, arecoline treatment disturbs the distribution of mitochondria, reduces A

SUBMITTER: Li WD 

PROVIDER: S-EPMC7377942 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature