Novel Mouse Model for Studying Hemostatic Function of Human Platelets.

ABSTRACT:

Objective

Platelets are critical to the formation of a hemostatic plug and the pathogenesis of atherothrombosis. Preclinical animal models, especially the mouse, provide an important platform to assess the efficacy and safety of antiplatelet drugs. However, these studies are limited by inherent differences between human and mouse platelets and the species-selectivity of many drugs. To circumvent these limitations, we developed a new protocol for the adoptive transfer of human platelets into thrombocytopenic nonobese diabetic/severe combined immune deficiency mice, that is, a model where all endogenous platelets are replaced by human platelets in mice accepting xenogeneic tissues. Approach and Results: To demonstrate the power of this new model, we visualized and quantified hemostat

SUBMITTER: Paul DS 

PROVIDER: S-EPMC7377966 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature