Evolution of viral quasispecies during SARS-CoV-2 infection.

ABSTRACT:

Objectives

Studies are needed to better understand the genomic evolution of the recently emerged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This study aimed to describe genomic diversity of SARS-CoV-2 by next-generation sequencing (NGS) in a patient with longitudinal follow-up for SARS-CoV-2 infection.

Methods

Sequential samples collected between January 29th and February 4th, 2020, from a patient infected by SARS-CoV-2 were used to perform amplification of two genome fragments-including genes encoding spike, envelope, membrane and nucleocapsid proteins-and NGS was carried out with Illumina® technology. Phylogenetic analysis was performed with PhyML and viral variant identification with VarScan.

Results

Majority consensus sequences were identical in

SUBMITTER: Jary A 

PROVIDER: S-EPMC7378485 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature