Detection of Antimicrobial Resistance Using Proteomics and the Comprehensive Antibiotic Resistance Database: A Case Study.

ABSTRACT:

Purpose

Antimicrobial resistance (AMR), especially multidrug resistance, is one of the most serious global threats facing public health. The authors proof-of-concept study assessing the suitability of shotgun proteomics as an additional approach to whole-genome sequencing (WGS) for detecting AMR determinants.

Experimental design

Previously published shotgun proteomics and WGS data on four isolates of Campylobacter jejuni are used to perform AMR detection by searching the Comprehensive Antibiotic Resistance Database, and their detection ability relative to genomics screening and traditional phenotypic testing measured by minimum inhibitory concentration is assessed.

Results

Both genomic and proteomic approaches identify the wild-type and variant molecular determinants

SUBMITTER: Chen CY 

PROVIDER: S-EPMC7378939 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature