Ontology highlight
ABSTRACT:
SUBMITTER: Lycette BE
PROVIDER: S-EPMC7379158 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Lycette Brynne E BE Glickman Jacob W JW Roth Samuel J SJ Cram Abigail E AE Kim Tae Hee TH Krizanc Danny D Weir Michael P MP
Journal of proteome research 20160823 9
We identified tryptic peptides in yeast cell lysates that map to translation initiation sites downstream of the annotated start sites using the peptide-spectrum matching algorithms OMSSA and Mascot. To increase the accuracy of peptide-spectrum matching, both algorithms were run using several standardized parameter sets, and Mascot was run utilizing a, b, and y ions from collision-induced dissociation. A large fraction (22%) of the detected N-terminal peptides mapped to translation initiation dow ...[more]