Washing or filtering of blood products does not improve outcome in a rat model of trauma and multiple transfusion.

ABSTRACT:

Background

Transfusion is associated with organ failure and nosocomial infection in trauma patients, which may be mediated by soluble bioactive substances in blood products, including extracellular vesicles (EVs). We hypothesize that removing EVs, by washing or filtering of blood products, reduces organ failure and improves host immune response.

Materials and methods

Blood products were prepared from syngeneic rat blood. EVs were removed from RBCs and platelets by washing. Plasma was filtered through a 0.22-μm filter. Rats were traumatized by crush injury to the intestines and liver, and a femur was fractured. Rats were hemorrhaged until a mean arterial pressure of 40 mm Hg and randomized to receive resuscitation with standard or washed/filtered blood products, in a 1:1:1 ra

SUBMITTER: Wirtz MR 

PROVIDER: S-EPMC7379301 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature