Optimization of isothermal amplification method for Mycobacteriumtuberculosisdetection and visualization method for fieldwork

ABSTRACT:

Background/aim

Tuberculosis is still one of the most contagious diseases around the world. Key factors of tuberculosis control are rapid diagnostic, efficient treatment, and prevention of contamination by surveillance and monitoring. However, culture is the gold standard method for laboratory diagnosis of tuberculosis; the results are several weeks to obtain. In order to prevent contamination of tuberculosis, diagnosis must be made in short time and treatment should be started as soon as possible. The aim of this study is to optimize the loop-mediated isothermal amplification (LAMP) method, which provides a much faster and more sensitive result than the polymerase chain reaction (PCR) method and allows the repli

SUBMITTER: Agel HE 

PROVIDER: S-EPMC7379476 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature