Proteomics analysis of Trichoplusia ni midgut epithelial cell brush border membrane vesicles.

ABSTRACT: The insect midgut epithelium is composed of columnar, goblet, and regenerative cells. Columnar epithelial cells are the most abundant and have membrane protrusions that form the brush border membrane (BBM) on their apical side. These increase surface area available for the transport of nutrients, but also provide opportunities for interaction with xenobiotics such as pathogens, toxins and host plant allelochemicals. Recent improvements in proteomic and bioinformatics tools provided an opportunity to determine the proteome of the T. ni BBM in unprecedented detail. This study reports the identification of proteins from BBM vesicles (BBMVs) using single dimension polyacrylamide gel electrophoresis coupled with multi-dimensional protein identification technology. More than 3000 proteins were a

SUBMITTER: Javed MA 

PROVIDER: S-EPMC7379565 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature