SimuSCoP: reliably simulate Illumina sequencing data based on position and context dependent profiles.

ABSTRACT:

Background

A number of simulators have been developed for emulating next-generation sequencing data by incorporating known errors such as base substitutions and indels. However, their practicality may be degraded by functional and runtime limitations. Particularly, the positional and genomic contextual information is not effectively utilized for reliably characterizing base substitution patterns, as well as the positional and contextual difference of Phred quality scores is not fully investigated. Thus, a more effective and efficient bioinformatics tool is sorely required.

Results

Here, we introduce a novel tool, SimuSCoP, to reliably emulate complex DNA sequencing data. The base substitution patterns and the statistical behavior of quality scores in Illumina sequencing data

SUBMITTER: Yu Z 

PROVIDER: S-EPMC7379788 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature