Engineering the transmission efficiency of the noncyclic glyoxylate pathway for fumarate production in Escherichia coli.

ABSTRACT:

Background

Fumarate is a multifunctional dicarboxylic acid in the tricarboxylic acid cycle, but microbial engineering for fumarate production is limited by the transmission efficiency of its biosynthetic pathway.

Results

Here, pathway engineering was used to construct the noncyclic glyoxylate pathway for fumarate production. To improve the transmission efficiency of intermediate metabolites, pathway optimization was conducted by fluctuating gene expression levels to identify potential bottlenecks and then remove them, resulting in a large increase in fumarate production from 8.7 to 16.2 g/L. To further enhance its transmission efficiency of targeted metabolites, transporter engineering was used by screening the C₄-dicarboxylate transporters and then strengthening

SUBMITTER: Chen X 

PROVIDER: S-EPMC7379832 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature