METROID: an automated method for robust quantification of subcellular fluorescence events at low SNR.

ABSTRACT:

Background

In cell biology, increasing focus has been directed to fast events at subcellular space with the advent of fluorescent probes. As an example, voltage sensitive dyes (VSD) have been used to measure membrane potentials. Yet, even the most recently developed genetically encoded voltage sensors have demanded exhausting signal averaging through repeated experiments to quantify action potentials (AP). This analysis may be further hampered in subcellular signals defined by small regions of interest (ROI), where signal-to-noise ratio (SNR) may fall substantially. Signal processing techniques like blind source separation (BSS) are designed to separate a multichannel mixture of signals into uncorrelated or independent sources, whose potential to separate ROI signal from noise has

SUBMITTER: Zoccoler M 

PROVIDER: S-EPMC7379836 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature