Characterization of Neospora Caninum Microneme Protein 26 and Its Potential Use as a Diagnostic Marker for Neosporosis in Cattle.

ABSTRACT: The apicomplexan parasite Neospora caninum causes neosporosis, an illness that leads to abortion or stillbirth in cattle, causing massive economic losses to the livestock industry. Rapid and viable diagnosis is the premise of prevention and control for neosporosis. In this study, we screened a new microneme protein 26 (NcMIC26) through western blot and mass spectrometry identification from the excretory secretion antigen (ESA) of N. caninum tachyzoites. NcMIC26 is subcellularly localized to the microneme of parasites. NcMIC26 is a specific antigen of N. caninum and has no cross-reaction with Toxoplasma gondii. Therefore, NcMIC26 has the potential to be a candidate diagnostic antigen for neosporosis. To test this hypothesis, recombinant NcMIC26 (rNcMIC26) was exp

SUBMITTER: Wang X 

PROVIDER: S-EPMC7380076 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature