Spontaneous and photosensitization-induced mutations in primary mouse cells transitioning through senescence and immortalization.

ABSTRACT: To investigate the role of oxidative stress-induced DNA damage and mutagenesis in cellular senescence and immortalization, here we profiled spontaneous and methylene blue plus light-induced mutations in the cII gene from λ phage in transgenic mouse embryonic fibroblasts during the transition from primary culture through senescence and immortalization. Consistent with detection of characteristic oxidized guanine lesions (8-oxodG) in the treated cells, we observed significantly increased relative cII mutant frequency in the treated pre-senescent cells which was augmented in their immortalized counterparts. The predominant mutation type in the treated pre-senescent cells was G:C→T:A transversion, whose frequency was intensified in the treated immortalized cells. Conversely, the

SUBMITTER: Caliri AW 

PROVIDER: S-EPMC7380187 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature