Harnessing synthetic biology to enhance heterologous protein expression.

ABSTRACT: The ability to express heterologous proteins in microbial hosts is crucial for many areas of research and technology. In most cases, however, successful expression and purification of the desired protein require fusion to another protein. To date, all fusion partners have been chosen from natural sequences, which evolved for other purposes, and may not be optimal fusion partners. However, the rise of synthetic biology and protein design make it possible to design and optimize fusion proteins using novel sequences that did not arise in nature. Here, we describe a series of De novo Expression Enhancer Proteins (DEEPs) that facilitate high-level expression and facile purification of heterologous proteins and peptides. To test the DEEP system, a de novo protein was fused to several target prot

SUBMITTER: Zarzhitsky S 

PROVIDER: S-EPMC7380667 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature