Nicotine Changes the microRNA Profile to Regulate the FOXO Memory Program of CD8⁺ T Cells in Rheumatoid Arthritis.

ABSTRACT: Objective: Smoking suppresses PD-1 expression in patients with rheumatoid arthritis (RA). In this study, we assess if smoking changed the epigenetic control over CD8⁺ T cell memory formation through a microRNA (miR) dependent mechanism. Methods: Phenotypes of CD8⁺ T cells from smokers and non-smokers, RA and healthy, were analyzed by flow cytometry. A microarray analysis was used to screen for differences in miR expression. Sorted CD8⁺ cells were in vitro stimulated with nicotine and analyzed for transcription of miRs and genes related to memory programming by qPCR. Results: CD27⁺CD107a^-CD8⁺ T cells, defining a naïve-memory population, had low expression of PD-1. Additionally, the CD27⁺

SUBMITTER: Wasen C 

PROVIDER: S-EPMC7381249 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature