Real-time imaging of polymersome nanoparticles in zebrafish embryos engrafted with melanoma cancer cells: Localization, toxicity and treatment analysis.

ABSTRACT:

Background

The developing zebrafish is an emerging tool in nanomedicine, allowing non-invasive live imaging of the whole animal at higher resolution than is possible in the more commonly used mouse models. In addition, several transgenic fish lines are available endowed with selected cell types expressing fluorescent proteins; this allows nanoparticles to be visualized together with host cells.

Methods

Here, we introduce the zebrafish neural tube as a robust injection site for cancer cells, excellently suited for high resolution imaging. We use light and electron microscopy to evaluate cancer growth and to follow the fate of intravenously injected nanoparticles.

Findings

Fluorescently labelled mouse melanoma B16 cells, when injected into this structure proliferated ra

SUBMITTER: Kocere A 

PROVIDER: S-EPMC7381511 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature