A high-throughput method to quantify feeding rates in aquatic organisms: A case study with Daphnia.

ABSTRACT: Food ingestion is one of the most basic features of all organisms. However, obtaining precise-and high-throughput-estimates of feeding rates remains challenging, particularly for small, aquatic herbivores such as zooplankton, snails, and tadpoles. These animals typically consume low volumes of food that are time-consuming to accurately measure.We extend a standard high-throughput fluorometry technique, which uses a microplate reader and 96-well plates, as a practical tool for studies in ecology, evolution, and disease biology. We outline technical and methodological details to optimize quantification of individual feeding rates, improve accuracy, and minimize sampling error.This high-throughput assay offers several advantages over previous methods, including i) substantially reduced time a

SUBMITTER: Hite JL 

PROVIDER: S-EPMC7381556 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature