A total closed chest sheep model of cardiogenic shock by percutaneous intracoronary ethanol injection.

ABSTRACT: To develop a reproducible and stable closed chest model of ischemic cardiogenic shock in sheep, with high survival rate and potential insight into human pathology. We established a protocol for multi-step myocardial alcoholisation of the left anterior descending coronary artery by percutaneous ethanol injection. A thorough hemodynamic assessment was obtained by invasive and non-invasive monitoring devices. Repeated blood samples were obtained to determine haemoglobin and alcohol concentration, electrolytes, blood gas parameters and cardiac troponin I. After sacrifice, tissue was excised for quantification of infarction and histology. Cardiogenic shock was characterized by a significant decrease in mean arterial pressure (- 33%), cardiac output (- 29%), dP/dt_max (- 28%), carotid

SUBMITTER: Rienzo M 

PROVIDER: S-EPMC7381645 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature