Specific ablation of CD4⁺ T-cells promotes heart regeneration in juvenile mice.

ABSTRACT: Unlike adult cardiomyocytes, neonatal cardiomyocytes can readily proliferate that contributes to a transient regenerative potential after myocardial injury in mice. We have recently reported that CD4⁺ regulatory T-cells promote this process; however, the role of other CD4⁺ T-cell subsets as well as CD8⁺ T-cells in postnatal heart regeneration has been less studied. Methods: by comparing the regenerating postnatal day (P) 3 and the non-regenerating P8 heart after injury, we revealed the heterogeneity of CD4⁺ and CD8⁺ T-cells in the myocardium through single cell analysis. We also specifically ablated CD4⁺ and CD8⁺ T-cells using the lytic anti-CD4 and -CD8 monoclonal antibodies, respectively, in juvenile mice

SUBMITTER: Li J 

PROVIDER: S-EPMC7381734 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature