Adiponectin and related C1q/TNF-related proteins bind selectively to anionic phospholipids and sphingolipids.

ABSTRACT: Adiponectin (Acrp30) is an adipokine associated with protection from cardiovascular disease, insulin resistance, and inflammation. Although its effects are conventionally attributed to binding Adipor1/2 and T-cadherin, its abundance in circulation, role in ceramide metabolism, and homology to C1q suggest an overlooked role as a lipid-binding protein, possibly generalizable to other C1q/TNF-related proteins (CTRPs) and C1q family members. To investigate this, adiponectin, representative family members, and variants were expressed in Expi293 cells and tested for binding to lipids in liposomes using density centrifugation. Binding to physiological lipids were also analyzed using gradient ultracentrifugation, liquid chromatography-mass spectrometry, and shotgun lipidomics. Interestingly, adipo

SUBMITTER: Ye JJ 

PROVIDER: S-EPMC7382265 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature