Genistein Represses HOTAIR/Chromatin Remodeling Pathways to Suppress Kidney Cancer.

ABSTRACT:

Background/aims

Genistein, a soy isoflavone, has been shown to have anti-cancer effects in various cancers including renal cancer. Long non-coding RNA, HOX transcript antisense RNA (HOTAIR), is involved in cancer progression and metastasis, such as renal cancer. Our aim was to investigate the effects of genistein on HOTAIR chromatin remodeling functions.

Methods

We used MTS assays and Transwell migration assays to study the effects of genistein on cell proliferation and migration respectively in human renal cell carcinoma (RCC) cell lines. We used Western blots to analyze SNAIL and ZO-1 expression. We performed chromatin immunoprecipitation (ChIP) assays to study recruitment of the polycomb repressive complex 2 (PRC2) to the ZO-1 promoter. We performed RNA immunoprecipitatio

SUBMITTER: Imai-Sumida M 

PROVIDER: S-EPMC7382546 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature