The reliability of immunoassays to detect autoantibodies in patients with myositis is dependent on autoantibody specificity.

ABSTRACT:

Objectives

In order to address the reliability of commercial assays to identify myositis-specific and -associated autoantibodies, we aimed to compare the results of two commercial immunoassays with the results obtained by protein immunoprecipitation.

Methods

Autoantibody status was determined using radio-labelled protein immunoprecipitation for patients referred to our laboratory for myositis autoantibody characterization. For each autoantibody of interest, the sera from 25 different patients were analysed by line blot (Euroline Myositis Antigen Profile 4, EuroImmun, Lübeck, Germany) and dot blot (D-Tek BlueDiver, Diagnostic Technology, Belrose, NSW, Australia). Sera from 134 adult healthy controls were analysed.

Results

Overall commercial assays performed reasonably

SUBMITTER: Tansley SL 

PROVIDER: S-EPMC7382594 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature