Stiffness-mediated mesenchymal stem cell fate decision in 3D-bioprinted hydrogels.

ABSTRACT:

Background

Hydrogels with tuneable mechanical properties are an attractive material platform for 3D bioprinting. Thus far, numerous studies have confirmed that the biophysical cues of hydrogels, such as stiffness, are known to have a profound impact on mesenchymal stem cell (MSC) differentiation; however, their differentiation potential within 3D-bioprinted hydrogels is not completely understood. Here, we propose a protocol for the exploration of how the stiffness of alginate-gelatin (Alg-Gel) composite hydrogels (the widely used bioink) affects the differentiation of MSCs in the presence or absence of differentiation inducing factors.

Methods

Two types of Alg-Gel composite hydrogels (Young's modulus: 50 kPa vs. 225 kPa) were bioprinted independently of porosity. Then, stiff

SUBMITTER: Liu Y 

PROVIDER: S-EPMC7382973 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature