Early Detection in a Mouse Model of Pancreatic Cancer by Imaging DNA Damage Response Signaling.

ABSTRACT: Despite its widespread use in oncology, the PET radiotracer ¹⁸F-FDG is ineffective for improving early detection of pancreatic ductal adenocarcinoma (PDAC). An alternative strategy for early detection of pancreatic cancer involves visualization of high-grade pancreatic intraepithelial neoplasias (PanIN-3s), generally regarded as the noninvasive precursors of PDAC. The DNA damage response is known to be hyperactivated in late-stage PanINs. Therefore, we investigated whether the SPECT imaging agent ¹¹¹In-anti-γH2AX-TAT allows visualization of the DNA damage repair marker γH2AX in PanIN-3s in an engineered mouse model of PDAC, to facilitate early detection of PDAC. Methods: Genetically engineered KPC (KRas^LSL.G12D/+; p53^LSL.R172H/+; PdxCre) m

SUBMITTER: Knight JC 

PROVIDER: S-EPMC7383084 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature