Cold Atmospheric Plasma Disarms M1 Protein, an Important Streptococcal Virulence Factor.

ABSTRACT: Cold atmospheric plasma (CAP) has been demonstrated to be a successful antiseptic for chronic and infected wounds. Although experimental work has focused on elucidation of the curative power of CAP for wound healing, the molecular mechanisms behind this ability are less understood. To date, the direct effect of CAP on the activity of microbial virulence factors has not been investigated. In the present study, we therefore examined whether CAP can modulate the detrimental activity of M1 protein, one of the most studied Streptococcus pyogenes virulence determinant. Our results show that CAP abolishes the ability of M1 protein to trigger inflammatory host responses. Subsequent mass spectrometric analysis revealed that this effect was caused by oxidation of Met81 and Trp128 located at the sub-

SUBMITTER: Persson ST 

PROVIDER: S-EPMC7383272 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature