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Sensor Array Based Determination of Edman Degradated Amino Acids Using Poly(p-phenyleneethynylene)s.

ABSTRACT: A cross-reactive optical sensor array based on poly(p-phenyleneethynylene)s (PPEs) determines Edman degraded amino acids. We report a sensor array composed of three anionic PPEs P1-P3, and their electrostatic complexes with metal ions (Fe2+ , Cu2+ , Co2+ ). We recorded distinct fluorescence intensity response patterns as "fingerprints" of this chemical tongue toward standard phenylthiohydantoin (PTH) amino acids-degradation products of the Edman process. These "fingerprints" were converted into canonical scores by linear discrimination analysis (LDA), which differentiates all of the PTH-amino acids. This array discriminates PTH-amino acid residues degraded from an oligopeptide through Edman sequencing. This approach is complementary to chromatography approaches which rely on mass spectrometry; our array offers the advantage of simplicity.

SUBMITTER: Zhang H

PROVIDER: S-EPMC7383564 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Sensor Array Based Determination of Edman Degradated Amino Acids Using Poly(p-phenyleneethynylene)s.

Zhang Hao H Wang Benhua B Seehafer Kai K Bunz Uwe H F UHF

Chemistry (Weinheim an der Bergstrasse, Germany) 20200529 35

A cross-reactive optical sensor array based on poly(p-phenyleneethynylene)s (PPEs) determines Edman degraded amino acids. We report a sensor array composed of three anionic PPEs P1-P3, and their electrostatic complexes with metal ions (Fe2+ , Cu2+ , Co2+ ). We recorded distinct fluorescence intensity response patterns as "fingerprints" of this chemical tongue toward standard phenylthiohydantoin (PTH) amino acids-degradation products of the Edman process. These "f ...[more]

PMID: 32181541

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