The transcription factor GATA10 regulates fertility conversion of a two-line hybrid tms5 mutant rice via the modulation of Ub_L40 expression.

ABSTRACT: The thermosensitive genic male sterile 5 (tms5) mutation causes thermosensitive genic male sterility in rice (Oryza sativa) through loss of RNase Z^S1 function, which influences ubiquitin fusion ribosomal protein L40 (Ub_L40 ) messenger RNA levels during male development. Here, we used ATAC-seq, combined with analysis of H3K9ac and H3K4me2, to identify changes in accessible chromatin during fertility conversion of the two-line hybrid rice Wuxiang S (WXS) derived from a mutant tms5 allele. Furthermore, RNA-seq and bioinformatic analyses identified specific transcription factors (TFs) in differentially accessible chromatin regions. Among these TFs, only GATA10 targeted Ub_L40 . Osgata10 knockout mutations, which resulted in low expression of Ub_L40 and

SUBMITTER: Jin J 

PROVIDER: S-EPMC7383616 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature